Fig 1: Cell death-inducing activity of wild-type and mutated NLP1 proteins expressed in melon. (a) The whole area of melon cotyledons was infiltrated with the Agrobacterium tumefaciens strains shown in Figure 1b. The photograph was taken at 5 days postinfiltration (dpi). (b) Quantitative analysis of necrotic lesion development in infiltrated melon cotyledons from (a). ImageJ was used for measuring the ratios of lesion area in infiltrated cotyledons by adjusting the colour threshold. Means and standard errors were calculated from three independent experiments. (c) Protein extracts at 5 dpi from melon cotyledons expressing the wild-type or mutated NLP1 proteins were analysed by immunoblotting (IB) using anti-HA (3F10; Roche) as the primary antibody and horseradish peroxidase-linked anti-rat IgG (#7077; Cell Signaling Technology) as the secondary antibody
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